Distributivity and Processivity in Multisite Phosphorylation Can Be Distinguished through Steady-State Invariants

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Distributivity and processivity in multisite phosphorylation can be distinguished through steady-state invariants.

Multisite protein phosphorylation and dephosphorylation are key cellular regulatory mechanisms but their system properties have been difficult to study in vivo and in vitro. Here we show by mathematical analysis that steady-state invariants enable the mechanism of the kinase or the phosphatase to be determined from steady-state measurements. Invariants exist when both enzymes act distributively...

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2007

ISSN: 0006-3495

DOI: 10.1529/biophysj.107.110866